Does a tbsp of butter in coffee break a fast and/or autophagy

[ladylyssa]

Darn it, my hubby used to work in a lab.

[grace2166]

A spectrophotometric assay for routine measurement of mammalian target of rapamycin activity in cell lysates.

HE Dekter, FP Romijn, WP Temmink, J van Pelt, JW de Fijter and NP Smit, Analytical biochemistry , Aug 2010

The mammalian target of rapamycin (mTOR) is an important mediator in the PI3K/AKT signaling pathway. mTOR is the target of immunosuppressive drugs, such as rapamycin and everolimus, that are used in transplant patients but also for the treatment of various cancers. We have developed a method for mTOR activity measurement in cell lysates that measures the phosphorylation of p70 S6 kinase by an enzyme linked immunosorbent assay (ELISA) protocol. Using an optimized lysis composition, activity could be measured in the peripheral blood mononuclear cells (PBMCs) isolated from blood. For the PBMCs, intra- and interassay variations of 7 and 10%, respectively, were found using one lot number of the kit. With different lot numbers, the interassay variation increased up to 21%. Activity remained constant for PBMC pool samples on storage for a period of more than 7 months. Activity could also be measured in CD3+ T-cells isolated from blood. In vitro experiments revealed maximum mTOR inhibition of 30% in PBMCs and 44% in T-cells. The in vitro inhibition in PBMCs could also be demonstrated by Western blotting. The mTOR activity measurements may be used to show in vivo inhibition in renal allograft patients during everolimus treatment and to study mTOR activity in various (tumor) cell types.

Huh???

[OldDoug]

Some good information in a related thread: Rosedale and mtor - how do we decrease it?

[Nursecamper]

Justin.Traer — I listened to the interview, amazing information. Some too nitty gritty for my understanding, but this is an exciting time to be alive. Also, I now have the answer to my question, ”How long of an extended fast is recommended for morbid obesity?” Thanks!!

[Chipmunk]

I’d agree with this opinion!

[Ketoning]

Interesting video on the effects of cream, MCT oil, and butter in coffee, as to whether or not it will break a fast.
Becky was not initially following a ketogenic diet, but Keith was, and had been for at least 7 months.
Keith’s results appeared to have been disappointing because he was looking for those magic numbers.
Becky’s numbers were very impressive.

My question (and theory) is, can Keith’s blood glucose and ketone numbers have been registering so low because he is more more fat adapted. If his body is utilizing ketones successfully, the “spillage” into his bloodstream may not accurately reflect his ability to utilize ketones.
I’m hoping there’s is a hail Mary here, because I have been doing ketogenic lifestyle for 1 month now. My blood ketones are all over the map-and showing very disappointingly low numbers (avg 0.7-1.0).
I am doing IF using my bulletproof coffee as a means to get thru the 16/8 fast. I’ve lost a whopping 3 lbs in a month, with a starting BF 26%, but I see a difference in how my clothing fit.

At 5’6” 168lbs, I’m just hovering over the healthy weight limit. I realize the scale doesn’t paint the bigger picture.
I’m also trying a low protein day twice a week to kickstart me into a higher ketosis range. I’m 56, and post menopause.
I’m not sure where (or if) I’m doing something to kick me out of ketosis, while showing low ketones in my bloodstream or if I’m just fat adapted and my bloodstream won’t always show high ketones.
Can one still be in ketosis and show blood ketones in the low range? Obviously weight and clothing fit will be a better judge of that.

[MarkGossage]

very disappointingly low numbers (avg 0.7-1.0).

Why do you find those disappointing? Perfectly good nutritional ketosis…

Can one still be in ketosis and show blood ketones in the low range?

Yours aren’t in a “low range” though

[Ketoning]

Hmmm, I’m under the assumption that a better reading should look more like (1.5-2.0). I’m never (and never was) in that range. If I’m in ketosis, it should reflect a bit more either on a scale or in my clothing. And neither are truly budging.