Anyone know: In this study what Fats were used?

(Mark Rhodes) #1


Is anyone familiar with this study? Before paying for it I would like to know WHAT fats were used on a HF diet. I am assuming that they used seed and vegetable oils. Yes, I do know it was a mouse study, lol.

(karen) #2

From the free page: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5417827/

All studies were approved by the Institutional Animal Care and Use Committee of the University of Washington. C57Bl/6J male mice (Jackson Labs, #000664) were housed (3–5 mice per cage) in a specific pathogen-free barrier facility in a temperature-controlled room (22 °C) with a 12-h light/dark cycle, and given free access to food and water. At 8 weeks of age, the mice were fed either a low-fat (4%) regular chow diet (Wayne Rodent BLOX 8604; Harlan Teklad Laboratory, Madison, WI), or a high-fat (60% calories from fat, Bioserve, Flemington, NJ, #F1850) diet and were analyzed at 16 weeks and 26 weeks of age.

So they were fed this:

It’s about 36% carb from sucrose, so once again focusing on fat when loading with sugar. :roll_eyes:

Lard, Sucrose, Casein, Maltodextrin, Mineral Mix, Vitamin Mix, DL-Methionine, Choline Chloride

ETA: I think this is the control diet:

54% carb

Dehulled soybean meal, wheat middlings, flaked corn, ground corn, fish meal, cane molasses, ground wheat, dried whey, soybean oil, brewers dried yeast, dicalcium phosphate, calcium carbonate, iodized salt, choline chloride, kaolin, magnesium oxide, ferrous sulfate, vitamin E acetate, menadione sodium bisulfite complex (source of vitamin K activity), manganous oxide, copper sulfate, zinc oxide, niacin, thiamin mononitrate, vitamin A acetate, vitamin D3 supplement, calcium pantothenate, pyridoxine hydrochloride, riboflavin, vitamin B
12
supplement, calcium iodate, folic acid, biotin, cobalt carbonate.

(karen) #3

ETA … so what happens if they just don’t Like it?

Jason Fung made me smile when he explained one problem with studies in humans; “you’ll be on this diet of powder x for three years. It’s chocolate flavor. … Do you like chocolate?”

(Mark Rhodes) #4

Thanks I couldn’t find it. I just wanted to know if this was a good HF diet or not before ordering the text. Seem pointless now. For some reason I could not access the full article. I thank you for this!!!

(You've tried everything else; why not try bacon?) #5

Teklad 8604 is a high-protein, low-fat food suitable for nursing rats and pups under the age of about four months (after that, the protein content needs to drop to 18%). Teklad blocks are one of the foods recommended to rat owners as a high-quality healthy diet (the others are made by Mazuri and Oxbow). The experience of rat owners is favorable; but the fat content is about half what I understand a mouse would require. My understanding is further that the protein content of 8604 would be excessive in adult mice; that high a level of dietary protein certainly causes problems in adult rats.

The problems with Bio-Serv F1850 have already been noted, so I will refrain.

The question I would like answered is why the researchers analyzed only epididymal fat. Couldn’t they do biopsies anywhere else? How do we know that reproductive tissue is representative of the rest of the body? What reasons dio we have for considering the observed changes deleterious?

(You've tried everything else; why not try bacon?) #6

By the way, if anyone knows of a decent ketogenic rat chow, I’d love to know about it.